Description
The Eagle Biosciences Apolipoprotein B ELISA Assay Kit employs the sandwhich enzyme immunoassay technique for the detection of Human Apolipoprotein B in human plasma, serum, and urine samples. Human Apolipoprotein B will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-Human Apolipoprotein B primary antibody binds to the captured protein. Following a washing to remove unbound substances, secondary antibody conjugated to Horseradise Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibody-enyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of Apolipoprotein B bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm. The concentration of Apolipoprotein B in the sample is then determined by comparing the O.D. of samples to the standard curve